ABSTRACT
Background and Aim: Contamination of food with enterotoxins of Staphylococcus aureus is a risk factor for public health. Because of lack of information on the contamination of the processed meat products including sausage and hams with the enterotoxins of Staphylococcus aureus, the present study was conducted to assess the presence of these toxins in the aforementioned products in Iran
Material and Methods: In this cross sectional study, 72 samples of sausage and ham were obtained from product storages of four active meat processing plants with different qualitative grades; including A, B, C and D in summer of 2015 in Isfahan Province, Iran. The qualitative grading of the meat processing plants was performed on the basis of the [pre requisite programs: PRPs] form approved by Food and Drug Administration of Ministry of Health in Iran. The scores of hygienic factors including [hygiene of workers], [production and processing], [washing, disinfection, cleaning] and [hazard identification and verification] of the aforementioned meat processing plants were determined according to the PRPs form
The meat processing plant with qualitative grades of A, B, C and D had the scores of 924, 825, 754 and 614, respectively. The hygienic grades of meat processing plants were [desirable], based on the PRPs form. The ELISA kite was used to detect the enterotoxins of Staphylococcus aureus
Results: The results showed lack of contamination of the processed meat products with enterotoxins of Staphylococcus aureus in all samp Is obtained from all of the factories with different qualitative grades
Conclusion: The result of the current study showed that the implementation of suitable designs in relation to the hygienic principles and the continuous surveillance of food inspectors of the Department of Food and Drug of Isfahan University of medical sciences could have a positive role in prevention of contamination of sausage and hams with enterotoxins of Staphylococcus aureus
ABSTRACT
Background: clostridium difficile [C. difficile] infection is one of the most important diseases in healthcare facilities and community. Ribotypes 027 and 078 are known as hyper- virulent strain of C. difficile in molecular study. PCR-ribotyping is a suitable method to interpret the relation of C. difficile isolated from food and hospital
Objectives: in the present study, the clostridim difficile binary toxin [cdtB] and ribotype pattern evaluated in toxigenic C. difficle isolated from beef
Methods: detection of cdtB in 12 toxigenic C. difficile [encoding tcdA and tcdB gene] isolated from 100 beef samples was determined through PCR. Afterwards, PCR-ribotyping was performed to examine the ribotype patterns of C. difficile
Results: cdtB gene was not detected in any positive isolate. Ten different patterns were observed in 12 toxigenic isolates. No similarity existed in the ribotypes of our study with ribotypes 027 and 078
Conclusions: albeit ribotyp 027 and 078 were not found in our study, the isolation of toxigenic C. difficile with new ribotypes in Iran may indicate the probable hazard of this bacterium in public health. Comprehensive research about C. difficile in different food sources is recommended on a national level